UCSD Layoff from Career Appointment: Apply by 4/24/17 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Eligible Disability Counseling and Consulting services (DCC) or Special Selection clients should contact their Vocational Rehabilitation Counselor for assistance.
Optic neuropathies, including glaucoma, are defined by the axonal injury and subsequent cell death of projection neurons in the retina called retinal ganglion cells (RGCs). The Welsbie Lab in the Department of Ophthalmology uses high-throughput RNAi- and CRISPR-based functional genomic screening in primary mouse RGCs and stem cell-derived human RGCs to elucidate and characterize the injury signal triggered by axon injury. The goal is to identify druggable signaling molecules that could serve as the target for a neuroprotective therapy.
The Lab Research Associate's role would be two-fold: 1. Assist with conducting wet lab and animal-based research, focusing on cell and molecular biology, under close technical supervision and guidance of the PI. 2. Assist with the organization and maintenance of the laboratory. The incumbent will assist in providing work direction and teaching laboratory procedures to undergraduate and graduate students, assist postdoctoral fellows and the work of laboratory technicians and students. In addition, the incumbent will provide the PI with support on personnel matters, audits, monitor lab budgets, monitor/coordinate lab safety issues (in collaboration with the UCSD Environmental Health and Safety Department and other governing safety agencies), assist with grant and publication preparation, ensure grant reporting progress is met, coordinate the organization and maintenance of lab equipment and supplies and interface with neighboring labs and collaborators. The Lab Research Associate will assist with the maintenance and organization of animals and the disposal of waste (nonhazardous, chemical and biological). As an active member of the research, the Lab Research Associate will assist in performing experiments and presenting at meetings/conferences.
Theoretical knowledge of molecular biology, cell biology, ophthalmology, biology or related field; or equivalent combination of education and experience.
Experience with PC and MAC, Microsoft Office Suite, scientific and research and database software.
Experience in accurate record keeping and ability to organize, analyze, and calculate data.
Excellent communication skills, verbal and written, to interact professionally and effectively in the work environment; and, to effectively and accurately document research procedures.
Experience or familiarity in the technologies required for analysis of tissues by histology, immunohistochemistry, and histochemistry.
Knowledge of basic lab safety requirements/procedures; EH&S regulations and methods for handling, clean-up, and waste disposal for biohazardous materials with the ability to train others in proper procedures.
Experience handling animals and animal tissue. Ability to follow appropriate protocols for animal research.
Knowledge of and experience with laboratory equipment including centrifuges, PCR machines, microscopes, tissue culture cabinets, incubators, flow cytometers, plate readers and gel electrophoresis equipment.
Familiarity with optic nerve crush procedures, intravitreal injections, retinal flatmount preparation, retinal ganglion cell isolation and/or viral production is desirable.
Demonstrated experience processing tissues and cells, cut sections, and performing histological staining procedures.
Experience with UCSD business offices, HR, EH&S, OPAFS, OCGA, procurement as well as working with online systems.
Must be willing to work occasional evening and weekends.
Must be willing to work with biohazardous material, viral vectors, human stem cells and rodents including mice and rats.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and ...five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.