Job Requisition Number: 26095. The Department of Molecular & Cell Biology (MCB) is home to Undergraduate and Graduate academic programs as well as Faculty Research performed by faculty organized in five divisions:
Biochemistry, Biophysics and Structural Biology Cell and Developmental Biology Genetics, Genomics and Development Immunology and Pathogenesis Neurobiology
The Research Technician will assist with experiments investigating the basic biology of interactions between bacterial pathogens (including M. tuberculosis and S. aureus) and mammalian hosts. It is anticipated that a broad range of techniques in molecular biology, biochemistry, and bacteriology will be used including DNA cloning/sequencing, growth and manipulation of mammalian cell lines, and growth and manipulation of M. tuberculosis, S. aureus, and potentially other bacteria. This position also includes duties related to keeping a laboratory functioning smoothly, including ordering, stocking, and laboratory maintenance.
•Mammalian cell culture. Specifically, culture of immortalized progenitor lines of neutrophils and macrophages and generation of new cell lines in which specific genes have been targeted using CRISPR. •Learn and carry out basic molecular/cell biology techniques such as DNA cloning, qPCR, transfection, lentivirus production to overexpress and silence gene expression in mammalian cell culture. •Biochemical techniques such as SDS-PAGE, affinity purification, western blotting. , •Assist other researchers in making technical determinations and observations based on the research. •Presentation of results at group meetings or in meetings with supervisor(s) •Coordinating vendors in procurement and maintenance of laboratory equipment. •Ordering/stocking laboratory supplies, preparation of lab stocks, laboratory maintenance. •Perform other duties as assigned•Experience with basic pipetting technique, molecular cloning and culture of mammalian cells •Ability to independently make solutions based on molarity and % composition
Education/Training: •BA/BS in biology or closely related science and knowledge of, or experience with, the basic techniques or methods required by the position; or an equivalent combination of education and experience. •Prior technician experience or undertaking independent research project as an undergraduate This position is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at: http://ucnet.universityofcalifornia.edu/labor/bargaining-units/rx/index.html
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