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Application Review Date The First Review Date for this job is: May 15, 2019
Departmental Overview The DuPage lab is pursuing a radically new strategy to circumvent both challenges by reprogramming the functionality of Regulatory T cells (Tregs) from an immunosuppressive to pro-inflammatory state specifically within the tumor microenvironment. The lab combines sophisticated genetic techniques, genomic analyses, and single cell assays to interrogate immune regulation in the settings of cancer and autoimmunity.
The lab is seeking a Staff Research Associate to investigate genes critical to the function of the T regulatory immune cell population in disease pathology, including autoimmunity and cancer.
Mouse colony management - determine what alleles must be genotyped for new mouse litters, harvest tail or blood samples from mice, isolate DNA from tissues, genotype the mice by PCR or flow cytometry, critically assess the results, prediction of genotype frequencies using rules of Mendelian genetics, update mouse genotype database. Capable of designing, testing and optimizing new primers for PCR genotyping of specific alleles. Able to determine antibody stains needed to determine genotype of mice by flow cytometric analysis. Experience injecting mice by sub cutaneous and intra peritoneal injection. Drug delivery by oral gavage. Sub cutaneous tumor inoculations and monitoring by measurement with calipers. EAE induction and monitoring of clinical score. Glucose monitoring for diabetes incidence.
Cell biological experiments - Harvest tissues from mice and generate single cell suspensions (from lymph nodes, spleen, thymus, tumors, tisssues). Culture of cells using sterile technique counting and propagating cells. Transfection of cells, viral infection of cells, antibody staining of cell surface and intracellular antigens and analysis of cells using FACSCalibur or LSRll flow cytometers. Experience sorting cells by FACS (i.e. use of FACSAria or equivalent).
Data analysis/ interpretation - daily record keeping in laboratory notebook (including the generation of written protocols for new assays learned), graphing cumulative data generated interpretation of data and drawing conclusions preparation for presentation at lab meetings. Attendance at weekly lab meetings. Background reading/research necessary to understand projects.
DNA manipulation/cloning - bacterial transformation, bacterial growth, plasmid purification, restriction enzyme digestion for analytical and cloning purposes, agarose gel purification of restricted bands ligation of DNA fragments. Independently design cloning strategies and troubleshoot cloning projects including the capacity to validate proper cloning.
Demonstrated record of organization, clear communication and excellent attendance.
Ability to follow instructions precisely.
Ability to reproducibly perform precision procedures.
Ability to work independently, having the initiative to research procedures and information, devising experiments and critiquing experimental results.
Ability to work collaboratively, learning and sharing techniques and knowledge with other members of the research team.
Ability to organize and perform several experiments in parallel on the same day.
Live mouse handling and manipulation (ie injections, bleeding, tagging)
Mouse necropsy and tissue harvesting.
Data analysis using software such as flowjo, Microsoft excel (or equivalent).
Molecular biology techniques including PCR and primer design, cloning by restriction digests and ligation of DNA plasmids agarose gel electrophoresis.
Cell culture techniques including sterile technique, media preparation, cell growth, transfection, retroviral production, and viral infection.
Antibody staining and flow cytometric analysis using LSRII or Fortessa instruments (experienced setting up these machines).
Bachelor's degree in Biology or a related science or an equivalent combination of education and experience.
Theoretical knowledge of immunology.
Research experience in a research lab (academic or industrial).
Experience organizing and determining mouse genotypes using mendelian genetics, PCR, and flow cytometry. Immune cell preparation from mouse blood or lymphoid tissues.
Experience setting up and using FACS (cell-sorting) machines.
Salary & Benefits Salary range (stepped rates): $47,100-$49,020, commensurate with experience.
For information on the comprehensive benefits package offered by the University visit:
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