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Application Review Date
The First Review Date for this job is: October 11, 2019
The Department of Molecular and Cell Biology is one of UC Berkeley's largest academic departments and is by several measures (including number of the faculty and students, budget, and assigned square feet), larger than many UCB colleges and schools. MCB is divided into five divisions: Biochemistry, Biophysics & Structural Biology (BBS); Cell & Developmental Biology (CDB); Genetics, Genomics & Development (GGD); Immunology & Pathogenesis (I&P); and Neurobiology (NEU). The teaching and research activities of the Department of MCB concern the molecular structures and processes of cellular life and their roles in the function, reproduction, and development of living organisms.
Organization, planning and preparation of the Genetics, Genomics and Development laboratory course (MCB140L) and Mammalian Neuroanatomy (MCB163L).
Responsible for the organization, planning and maintenance of the teaching laboratory, ensuring that all required materials are available and functioning properly.
Using the course schedule, general protocols and student manual, determine the necessity of and perform back-up experiments to provide students with data for analysis in the event that the student experiment fails and, in addition, determine the quantity and optimal ordering time for specific biological and chemical reagents.
These duties include but are not limited to: mapping DNA, cloning a gene, genetic screen, DNA sequence analysis, mutation induction, mutations and suppression, complementation, , transposon mutagenesis, tissue specific expression, RNAi experiments in C. elegans, fluorescence imaging, and genetic suppression and complementation experiments, protein expression and purification in yeast.
Develop human/yeast translational experiments using yeast cells.
Make up a wide range of complex solutions, buffers, and media.
Initiate cultures of bacteria and yeast, test them, and expand cultures used for class experiments.
Set up, calibrate, maintain and trouble shoot electronic /physiological equipment (amplifiers, oscilloscopes, manipulators, plotters, microelectrode holders, stimulators).
Test equipment prior to and after student usage.
Replace and coordinate repair of defective equipment.
Perform experiments in Immunohistochemistry on mouse brain slices, tissue preparation of mouse brain slices (perfusions in mice, slicing fixed brains using a microtome) and staining.
Work with various organisms such as mice, c. elegans, Drosophila and earthworms.
Assist GSIs and students in trouble shooting or resolving technical problems during class.
Responsible for steps of experiments carried out between class sessions.
Set up and troubleshoot classroom computers including: Ethernet connection, printing, file sharing, etc.
Be familiar with programs used by the course including but not limited DNA Strider, PyMOL, and ImageJ.
Maintain records of class experimental data from semester to semester to provide quality control and continuity of instruction.
Adapt and modify procedures used in the teaching laboratory as new techniques are developed.
Revise pep manual and lab manuals to reflect procedural changes as well as to promote efficiency and orderly function in classrooms.
Work with faculty to develop new experimental protocols.
In consultation with faculty, make decisions regarding preparatory work and pilot experiments.
Recommend procedural changes to faculty and GSIs to optimize experimental results.
Develop and standardize protocols for Genetic, Neuroanatomy and Molecular Biology to establish work standards, streamline planning and purchasing processes, and keep protocols for the teaching laboratories abreast of current practice in the research laboratories.
Participation in teaching.
Assist faculty members in charge of the course with technical aspects of instruction by demonstrating and explaining new techniques to GSIs and students.
During GSI meetings and laboratory periods, answer questions, replenish supplies, and trouble shoot problems.
Assist GSIs and students in identifying and correcting causes of unsuccessful experiments and participate in experimental design of lab exercises.
Provide safety training for GSIs.
Give safety talk to students on first day of instruction.
Modify and standardize methodologies specific to experimental design of lab.
Pre-test equipment, reagents and techniques and run pilot experiments to determine optimum conditions to be used and produce back-up data for classroom analysis in the event that student experiments fail.
Implement course Website, posting handouts, office hours and other materials as requested by faculty.
Assist in preparation of the student lab manual.
Assist GSIs and students in resolving technical problems.
Demonstrate techniques to GSIs, who, in turn, demonstrate to students.
Responsible for steps of experiments carried out between class sessions.
Organize student work space in classroom to promote efficient and orderly performance.
Innovation and Development:
Test and pilot new procedures in Genetics, Genomics, and Development, Neuroanatomy, and molecular biology. Incorporate these modified procedures into student experimental protocols.
Utilize different methods of investigation including library research, consulting research lab personnel, and experimental trials using carefully controlled techniques and appropriate modifications.
Review inventory and order/procure supplies in a timely manner.
Ensure that supplies and equipment are present in the classroom in the required quantities and in working condition for each laboratory session.
In consultation with supervisor, recommend the replacement of obsolete teaching equipment and the acquisition of new equipment.
Research and evaluate the model types that best fit the needs of the course.
Monitor classroom conditions to ensure they are kept clean and the laboratory practices are in compliance with campus EH&S regulations.
Organize work and storage space to promote efficient and orderly function.
Do a yearly inventory of supplies and equipment including chemicals.
Update computer inventory of freezers, refrigerators, chemical cabinets, etc.
Participate in year-end clean up and maintenance projects including the once yearly disposal of hazardous waste through EH&S.
Coordinate the waste pick-up with other divisional teaching laboratories if feasible.
Maintain records and monitor the cost of waste disposal.
Work with supervisor and staff to ensure that safety practices in divisional teaching laboratories are in compliance with Cal OSHA and campus EH&S standards.
Coordinate yearly Lab Self- Survey, and provide copies of survey to the Li Ka Shing (LKS) and Valley Life Science (VLSB) Building Managers.
Participate in emergency response planning and drill.
Maintain and replenish emergency supplies for work unit located in LKS and VLSB buildings.
Implement the Injury and Illness Prevention Program (IIPP) in departmental teaching laboratories.
Maintain and implement the Chemical Hygiene plan, conduct periodic safety inspections, and maintain training documentation.
As senior staff research associate for the Genetic and Neuroanatomy teaching laboratories, train Laboratory Assistant and Staff Research Associate I in tasks associated with course preparation.
Plan and schedule work assignments on a weekly basis, and follow up on the results.
Other duties as assigned including but not limited: implement unit/course website and help unit with minor computer problems/issues.
The resource person in computer technology for this lab teaching unit.
Bachelor degree in Genetics, Genomics, and Development; Neurobiology; Molecular and Cell Biology; or a related field; or equivalent combination of education and experience.
Demonstrated advanced knowledge of and extensive skills in a combination of neurobiology, electrophysiology, molecular biology, genetics, genomics, and development.
Demonstrated experience working with electrophysiology instrumentation, Immunohistochemistry, tissue preparation (perfusions, cryostat slicing) and staining.
Demonstrated experience with and ability to perform the following techniques unassisted from provided protocols, and trouble shoot problems when needed, with results of adequate quality for use in class: maintenance and propagation of E. coli, DNA/RNA preparations, protein purification, , agarose and acrylamide-gel electrophoresis, Western Blot, optical/video microscopy, fluorescent antibody staining, image capture, DNA restriction digests, transformation, gene cloning, genetic screen, DNA sequence analysis, isolation, characterization , and complementation of auxotrophic mutation of yeast, mutation induction, mutations and suppression, RNAi experiments in C. elegans, fluorescence imaging, and genetic suppression and complementation experiments. PCR and electrophysiology techniques. Familiar with some or all of the following systems: C. elegans, yeast, mouse and drosophila systems.
Experience in using and maintaining a wide variety of instruments and equipment, including spectrophotometers, oscilloscopes, thermal cyclers, light/fluorescence microscopes, and centrifuges; and ability to clearly and concisely explain their use to graduate and undergraduate students.
Proficiency with computer and information management systems, including but not limited: Word, Excel, Photoshop software, and scientific program used by the course.
Demonstrated independent judgment, critical thinking, and strong organizational and analytical skills. Strong problem solving skills and the ability to define issues clearly and propose suitable alternatives when primary activities do not go as planned.
Effective oral and written communication skills, and ability to develop and maintain cooperative, effective working relationships with faculty, students, supervisors, and team members. Ability to consistently exercise a high degree of professionalism in relating to students, graduate student instructors, faculty, and co-workers.
Demonstrated initiative, flexibility, resourcefulness, and creative approaches to unique problems, as well as an understanding of the wider context in which these problems must be addressed.
Demonstrated understanding and ability to stay abreast, of rapidly changing technology and tools, and the ability to effectively forecast and incorporate emerging technological advances into the course planning process.
Ability to work well under time pressure.
Advanced Degree in Genetics, Genomics, and Development; Neurobiology; Molecular and Cell Biology; or a closely related field strongly preferred.
Strong computer science background.
Salary & Benefits
This is an exempt, monthly paid position. Annual salary is commensurate with experience.
For information on the comprehensive benefits package offered by the University visit:
The University of California was chartered in 1868 and its flagship campus - envisioned as a "City of Learning" - was established at Berkeley, on San Francisco Bay. Today the world's premier public university and a wellspring of innovation, UC Berkeley occupies a 1,232 acre campus with a sylvan 178-acre central core. From this home its academic community makes key contributions to the economic and social well-being of the Bay Area, California, and the nation.